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Recent publications demonstrate how VeraPrep (VeraBIND) Biotin provides a fast and reliable solution to detect and neutralize biotin interference in immunoassays, ensuring accurate results in clinical testing—an essential tool for lab researchers concerned with minimizing diagnostic errors.

Validation of a Commercial Reagent for the Depletion of Biotin from Serum/Plasma: A Rapid and Simple Tool to Detect Biotin Interference with Immunoassay Testing

J Appl Lab Med. 2021 Jul 7;6(4):992-997. doi: 10.1093/jalm/jfab022.

https://academic.oup.com/jalm/article/6/4/992/6256241

This study evaluates the effectiveness of VeraPrep Biotin, a commercial reagent used to deplete biotin from serum and plasma samples to prevent interference with immunoassays. The researchers sought to verify that VeraPrep Biotin does not impact analyte recovery in biotin-free control samples and can effectively remove biotin from both biotin-spiked samples and those from individuals who ingested biotin supplements. The results showed that over 95% of control samples maintained analyte recovery between 90-110% post-treatment, indicating that the reagent did not affect results. Additionally, VeraPrep Biotin successfully reduced biotin levels to below interference thresholds, demonstrating its utility as a reliable and easy-to-use tool for detecting and mitigating biotin interference in clinical testing.

Neutralization of biotin interference: preliminary evaluation of the VeraTest Biotin, VeraPrep Biotin and BioT-Filter

Clin Chem Lab Med. 2020 Jul 28;58(8):e130-e133. doi: 10.1515/cclm-2019-1121.

https://pubmed.ncbi.nlm.nih.gov/32092039/

This study evaluates the effectiveness of different methods to neutralize biotin interference in immunoassays, which can cause inaccurate clinical results. The researchers compared four methods: streptavidin beads, VeraPrep Biotin, VeraTest Biotin, and the BioT-Filter, using samples from healthy volunteers who consumed a single 100 mg dose of biotin. Both streptavidin beads and VeraPrep Biotin effectively reduced biotin levels to non-interfering concentrations, showing mean elimination rates of 99.95%. The BioT-Filter and a 24-hour washout period were less effective. The study highlights the need for reliable tools to detect and mitigate biotin interference in clinical laboratories to ensure accurate patient care.

Prevalence of Detectable Biotin in Five US Emergency Department Patient Cohorts

Clin Biochem. 2021 Jul:93:26-32. doi: 10.1016/j.clinbiochem.2021.03.009.

https://pubmed.ncbi.nlm.nih.gov/33771568/

This study aimed to estimate the prevalence of biotin supplementation among emergency department patients in the U.S. by screening 7,118 serum or plasma samples from five medical centers. Using an Abbott ARCHITECT Biotin assay, 4.1% of the samples showed biotin concentrations ≥10 ng/mL, with variation between 2.0% and 5.4% across different sites. Confirmation testing through LC-MS/MS revealed that 1.3% of samples contained detectable levels of biotin and its metabolites, bisnorbiotin and biotin sulfoxide. The study highlights the significance of biotin interference in laboratory diagnostics, particularly in emergency settings where biotin levels can lead to erroneous test results, potentially affecting patient care.