VeraBIND tTG Antibody Assay

Advanced Detection of tTG Antibodies for Research

The VeraBIND™ tTG Antibody Assay (RUO) is a cutting-edge, bead-based fluorescent immunoassay designed for the multiplex detection of total IgA and IgG antibodies against human tissue transglutaminase (tTG) in serum, plasma, saliva, or saline oral rinse samples. This semi-automated assay offers precise detection and quantification of these antibodies, making it an essential tool for researchers investigating gluten exposure and related immunological responses. 

Key Features:

  • Multiplex Detection:
    Detects both IgA and IgG antibodies against tTG simultaneously, enhancing the efficiency and accuracy of your research.

  • Advanced Interference Removal:
    Utilizes proprietary VeraBIND Clean Beads to remove a wide range of endogenous human immunoglobulin interferences, ensuring cleaner samples and more reliable results.

  • Customizable and Quantitative:
    Offers the option to use the VeraBIND Triplex Antibody Calibrator Set for the quantification of IgA and IgG antibodies, allowing for detailed and accurate analysis.

  • High Sensitivity and Specificity:
    Proven to deliver 100% sensitivity and specificity for detecting tTG antibodies after pre-treatment with VeraBIND Clean Beads, ensuring the highest level of accuracy in your research.

How It Works

  1. Sample Cleaning:
    Serum, plasma, saliva, or saline oral rinse samples are treated with VeraBIND Clean Beads to remove non-specific immunoglobulins and other interferences.

  2. Antibody Capture:
    The treated samples are then incubated with tTG antigen-coated VeraBIND Capture Beads, allowing for the specific capture of tTG antibodies.

  3. Detection and Measurement:
    After washing to remove non-specific antibodies, the captured tTG antibodies are detected using a duplex fluorescent conjugate. The results can be quantified using the optional Triplex Antibody Calibrator Set.

Applications

  • Celiac Disease Research:
    Although not intended for diagnostic use, this assay is valuable in studying the presence and persistence of tTG antibodies following gluten exposure.

  • Immunology Studies:
    Ideal for research into autoimmune responses related to gluten intake and other related conditions.

  • Assay Development:
    Supports the development of new assays for detecting and quantifying antibodies against tissue transglutaminase.

Kit Includes

  • VeraBIND Clean Beads 

  • 25X Wash Buffer 

  • Phosphate Buffer Saline 

  • Celiac tTG Duplex Conjugate 

  • Elution Buffer 

  • Neutralization Buffer 

  • tTG Antigen-Coated VeraBIND Capture Beads 

Materials Required But Not Included

  • BioPlex 2200 Celiac IgA Control Set (BIO-RAD, Reference Number 663-2330) 

  • BioPlex 2200 Celiac IgG Control Set (BIO-RAD, Reference Number 663-2230) 

  • Optional: VeraBIND™ Triplex Antibody Calibrator Set (Part Number 400092) 

  • Vortex mixer

  • Laboratory rocker

  • Multi-channel pipettes

  • Well plates

  • Plate magnets

  • Fluorescent reader 

Ordering Information

  • Product Name: VeraBIND™ tTG Antibody Assay

  • Catalog Number: 500100

Regulatory Status

For Research Use Only. Not for use in diagnostic procedures.